This study assessed the effects of population growth on investments opportunities in Calabar South Local Government Area of Cross River State, Nigeria. The study adopted the survey design, the sample size for the study were 210 respondents, the instrument of data collection was the questionnaire and the data collected was analyzed using the chi-square technique. From the chi-square result, it was revealed that there is a significant effect of population growth on investment opportunities in transportation businesses in Calabar South Local Government Area of Cross River State and there is a significant effect of population growth on investment opportunities in restaurant businesses in Calabar South Local Government Area of Cross River State. Based on the findings, the study recommended that the population growth rate in Cross River State should be reduced so as to stimulate socio-economic development in terms of more investment opportunities and population considerations should be taken into account at all levels of decision making. Again, the government should create a conducive investment climate for businesses in terms of reduction in taxation so that investments can thrive and government should create enabling environment that will facilitate savings, investment, innovation, entrepreneurship and technical know-how, among others.